Can Fluo-200 fluorometer judge the purity of the sample?
No, the fluorometer needs to be equipped with a fluorescence kit, which can only give the concentration of the sample. Because it is not measured by absorbance, it cannot give the purity judgment of A260/A280. If purity data is required, micro spectrophotometer can be used for testing.
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When the sample is placed on the base for a long time, evaporation may occur, and bubbles may be introduced during a test, resulting in inaccurate measurement. Therefore, we recommend that the sample be loaded again each time. -
The purified protein can be directly measured using A280 mode. Select the protein sample type (BSA, IgG, lysozyme) from the drop-down list to determine the most appropriate extinction coefficient. For the unpurified protein, the colorimetric method can be selected, and the standard curve can be established for measurement with the kit (BCA, Bradford, Lowry).
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Yes, the kit used on the Fluo-200 is open. Most manufacturers' kits can be directly tested by calibrating only two standard sample points. If you are worried about the error of the kit, you can also rebuild the standard curve by preparing multiple standard sample points.
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No, the fluorescence function of Feyond-A300 uses the filter mode for wavelength selection, and spectral scanning cannot be performed. If the wavelength required for the experiment is not within the bandwidth of the standard filter, the required filter needs to be customized.
