How to measure purified and unpurified proteins with a micro spectrophotometer?

Author: vch12331545

Published on: 2022-06-06 21:20

Classification: Common problem

The purified protein can be directly measured using A280 mode. Select the protein sample type (BSA, IgG, lysozyme) from the drop-down list to determine the most appropriate extinction coefficient. For the unpurified protein, the colorimetric method can be selected, and the standard curve can be established for measurement with the kit (BCA, Bradford, Lowry).

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